MRG QuickNotes ©1998
M Do not turn PC off.
MM Do not change any of the program or computer default settings.
MMM NEVER Download anything to the polymer science computers.
Importing a file:
- Pull down File menuWorking up the spectrum:
- Select Import/Export
- At Convert window- Select BRUKER
- Click Import
- Select data file (may need to select "All files (*.*)")
- Click OK- At File Import window
- Change name of file (if necessary)
- Under "Output File Type select Spectra (*.SPC) & click OK
- Select Complex Output & click OK
- Pull down File menuAnd that's all there is to it! Tough, huh?
- Select Open
- Select desired data file in SPC format & click OK
- Pull down Arithmetic menu
- Select Do Program
- Select nmrapps.ab and click OK
- Click Apodize
- Select Matched exponential and click OK
- When asked for a line broadening value:- For Proton spectra- Click FFT- Click OK- For Carbon 13 spectra
- Use + and - keys on number pad to make green line "fit" FID, then hit enter.- Type 2.7 and click OK
- Click Zero order
- Use + and - keys on the number pad to make baseline level (in case of 13C spectra, press F4 to flip 180o) then press enter.
- Click Done
- Click Disk save
- Click X-axis
- Click Select ref
- Choose reference peak on spectra (chloroform, TMS, etc)
- Input appropriate chemical shift in PPM (CD3Cl - 77.26, TMS - 0)
- Click OK
- Click Quit
All spectra should have the same limits so that they can easily be compared. Here is how to solve that problem.
- Pull down View menuSometimes, you might like to show two or more spectra on one plot. Here's how to do that.
- Select XYZ Settings
- Input desired left and right X limits- for 1H spectra - left = 10 right = -0.5 (unless spectrum- Click OK
- for 13C spectra - left = 200 right = 0 exceeds these limits)
- Work up all spectra as above
- Pull down View menu
- Select Trace Mode
- Select Stacked
- Click OK